| Molecular Formula | C257H381N73O83S7 |
| Molar Mass | 6200Da±600 |
| Appearance | Form powder, color white to off-white |
| Storage Condition | -20°C |
| Physical and Chemical Properties | Chemical properties Human epidermal growth factor has good acid and thermal stability; it is easily hydrolyzed by protease. pI4.6. Settlement constant 1.25s. It can be stored for a long time at -20 ℃. The three disulfide bonds are difficult to pair correctly. After mismatching, the protein molecule can be refold and reduce the activity or disappear. |
| Use | The application is suitable for the treatment of oral ulcer, erosion, scald, burn and the like, and the combination of large dose of human epidermal factor and toxin in the treatment of glioma, breast cancer and skin cancer has achieved certain curative effect, especially for squamous cell skin cancer curative effect is remarkable. |
| WGK Germany | 3 |
| RTECS | KB4518000 |
| FLUKA BRAND F CODES | 10-21 |
| Raw Materials | TRIS BORATE EDTA BUFFER, 10X, DNASE, RNASE AND PROTEASE FREE, PH 8.3, FOR MOLECULAR BIOLOGY |
| Reference Show more | 1. Huang saijin [1], Yin Aiwu [1], Liu Fangyu [2], etc. Effect of Portulaca oleracea L. On ethanol-induced gastric ulcer in rats [J]. Natural Products Research and Development, 2016(28):781-785. Note: For some products, our company can only provide some information, we do not guarantee the authority of the information provided, only for the reference of the customer. storage conditions: -20 ℃ residual moisture ≤ 3.0% endotoxin ≤ 1EU / 1 ug |
overview
EGF,INCI, called oligopeptide 1(Human oligopeptide-1), is a polypeptide containing 53 amino acid residues, with a size of 6.2 kDa and three disulfide bonds. EGF is distributed in a variety of tissues and body fluids and is a growth factor secreted by humans themselves. EGF has a vital effect on wound healing and organ formation. It can stimulate the proliferation, differentiation and survival of various epidermal epithelial cells; promote the growth and migration of keratinocytes, and promote the proliferation of fibroblasts.
production method
Extraction process using submandibular gland as raw material
minch, extract submandibular gland to remove fat and fascia, twist into chyme, add 0.05mol/L HAc liquid to stir and extract, centrifuge to collect the extract.
submandibular gland [minced] & rarr; Mi Zhuang [0.05 mol/L HAc solution] & rarr; Extract
adsorption and elution CMC is added to the extract, placed in a refrigerator, adsorbed, washed with pH 3.9 buffer, and eluted with pH 7.0 buffer.
extract [CMC]& rarr;CMC adsorbate [buffer] & rarr;[pH7.0] eluent
DEAE is used for secondary adsorption and elution to adsorb eluent, and impurity protein is washed with pH7.0 buffer solution. 0.2mol/L HCl and NaCl are used for elution respectively to collect eluent.
eluent [DEAE]& rarr;DEAE adsorbate [0.2 mol/L HCl, NaCl]& rarr; Eluate
freeze-drying and refining freeze the eluent into dry powder, then dissolve the freeze-dried powder, put it on a SephadexG-75 column, collect the effluent and refine it, and freeze-dry it to make a fine product.
eluent [lyophilized] & rarr; Freeze-dried powder [SephadexG-75 column] & rarr;EGF boutique.